yqfD

168

SigE-dependent sporulation gene, similar to UDP-glucose 4-epimerase

Locus: BSU_25350

Molecular weight: 45.57 kDa

Isoelectric point: 10

Protein length: 398 aa

Gene length: 1197 bp

Function: sporulation

Product: unknown

Essential: no

Synonyms: yqfD

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG5872 (Galperin et al., 2021)

Gene

Coordinates: 2,615,452 → 2,616,648

Phenotypes of a mutant

• defect in sporulation PubMed; some reduction in SigG activity and production of small spores PubMed

The protein

Structure

• P54469 (AlphaFold)

Localization

• polar (Septum) PubMed

Expression and Regulation

Operons

• Genes: yqfC-yqfD

  Description: PubMed

  Regulation

  • expressed early during sporulation in the mother cell (SigE, SpoIIID) PubMed

  Regulatory mechanism

  • SpoIIID: repression, PubMed, in spoIIID regulon

  Sigma factors

  • SigE: sigma factor, PubMed, in sigE regulon

Biological materials

Mutant

• MGNA-C444 (yqfD::erm), available at the NBRP B. subtilis, Japan
• BKE25350 (ΔyqfD::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTTCACAACATTTCCCCCTC,  downstream forward: _UP4_CCTATTGTCAGGGAGACTGA
• BKK25350 (ΔyqfD::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTTCACAACATTTCCCCCTC,  downstream forward: _UP4_CCTATTGTCAGGGAGACTGA

References

Loading