dgkA

168

undecaprenol kinase

Locus: BSU_25310

Molecular weight: 12.40 kDa

Isoelectric point: 7.13

Protein length: 123 aa

Gene length: 372 bp

Function: cell wall biosynthesis

Product: undecaprenol kinase

Essential: no

E.C.: 2.7.1.107

Synonyms: dgkA, yqxF

Genomic Context

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List of homologs in different organisms, belongs to COG0818 (Galperin et al., 2021)

Gene

Coordinates: 2,611,456  2,611,827

Phenotypes of a mutant

• inactivation of ''dgkA'' reduces sporulation efficiency to 33.7% that of wild type cells PubMed

The protein

Catalyzed reaction/ biological activity

• ATP + di-trans,octa-cis-undecaprenol --> ADP + di-trans,octa-cis-undecaprenyl phosphate + H⁺ (according to UniProt)

Protein family

• Bacterial diacylglycerol kinase family (single member, according to UniProt)

Structure

• P19638 (AlphaFold)

Localization

• cell membrane at the septum PubMed

Biological materials

Mutant

• BKE25310 (dgkA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTTGAAGAATCGATTCAGCT,  downstream forward: _UP4_TAATGGCTGAAAATTCTTAC
• BKK25310 (dgkA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTTGAAGAATCGATTCAGCT,  downstream forward: _UP4_TAATGGCTGAAAATTCTTAC

References

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