spoIIIAA

168

AAA protease, required for SigG activation

Locus: BSU_24430

Molecular weight: 34.14 kDa

Isoelectric point: 8.18

Protein length: 307 aa

Gene length: 924 bp

Function: activation of SigG

Product: AAA protease

Essential: no

Synonyms: spoIIIAA

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG3854 (Galperin et al., 2021)

Gene

Coordinates: 2,536,690  2,537,613

The protein

Structure

• Q01367 (AlphaFold)

Localization

• membrane protein PubMed

Expression and Regulation

Operons

• Genes: yqhV-spoIIIAA-spoIIIAB-spoIIIAC-spoIIIAD-spoIIIAE-spoIIIAF-spoIIIAG-spoIIIAH

  Description: PubMed

  Regulation

  • both promoters: expressed during sporulation in the mother cell (SigE) PubMed, switched off by SpoIIID PubMed

  Regulatory mechanism

  • SpoIIID: repression, PubMed, in spoIIID regulon

  Sigma factors

  • SigE: sigma factor, PubMed, in sigE regulon

• Genes: spoIIIAA-spoIIIAB-spoIIIAC-spoIIIAD-spoIIIAE-spoIIIAF-spoIIIAG-spoIIIAH

  Description: PubMed

  Regulation

  • both promoters: expressed during sporulation in the mother cell (SigE) PubMed, switched off by SpoIIID PubMed

Biological materials

Mutant

• BKE24430 (spoIIIAA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAACAGAGCCTCCTCCTTTC ,  downstream forward: _UP4_AATGTGCTGTCTAGGACGACG
• BKK24430 (spoIIIAA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAACAGAGCCTCCTCCTTTC ,  downstream forward: _UP4_AATGTGCTGTCTAGGACGACG

References

Reviews

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Original Publications

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