spoIIIAF

168

component of the SpoIIIA-SpoIIQ type III secretion system residing in the forespore membrane, required for SigG activation

Locus: BSU_24380

Molecular weight: 23.14 kDa

Isoelectric point: 4.95

Protein length: 206 aa

Gene length: 621 bp

Function: activation of SigG

Product: component of a type III secretion system

Essential: no

Synonyms: spoIIIAF

Genomic Context

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List of homologs in different organisms, belongs to COG5827 (Galperin et al., 2021)

Gene

Coordinates: 2,533,692  2,534,312

The protein

Structure

• 6DCS (PDB) PubMed
• P49783 (AlphaFold)

Localization

• membrane protein PubMed

Expression and Regulation

Operons

• Genes: yqhV-spoIIIAA-spoIIIAB-spoIIIAC-spoIIIAD-spoIIIAE-spoIIIAF-spoIIIAG-spoIIIAH

  Description: PubMed

  Regulation

  • both promoters: expressed during sporulation in the mother cell (SigE) PubMed, switched off by SpoIIID PubMed

  Regulatory mechanism

  • SpoIIID: repression, PubMed, in spoIIID regulon

  Sigma factors

  • SigE: sigma factor, PubMed, in sigE regulon

• Genes: spoIIIAA-spoIIIAB-spoIIIAC-spoIIIAD-spoIIIAE-spoIIIAF-spoIIIAG-spoIIIAH

  Description: PubMed

  Regulation

  • both promoters: expressed during sporulation in the mother cell (SigE) PubMed, switched off by SpoIIID PubMed

Biological materials

Mutant

• BKE24380 (spoIIIAF::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TAAAAAACTCATTTGCCTGC,  downstream forward: _UP4_ATGGAAGGCGGGGAGAGTGT
• BKK24380 (spoIIIAF::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TAAAAAACTCATTTGCCTGC,  downstream forward: _UP4_ATGGAAGGCGGGGAGAGTGT

References

Reviews

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Original Publications

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