yqiK

168

cytoplasmic glycerophosphodiester phosphodiesterase

Locus: BSU_24180

Molecular weight: 26.81 kDa

Isoelectric point: 6.43

Protein length: 243 aa

Gene length: 732 bp

Function: unknown

Product: cytoplasmic glycerophosphodiester phosphodiesterase

Essential: no

Synonyms: yqiK

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0584 (Galperin et al., 2021)

Gene

Coordinates: 2,514,169 → 2,514,900

The protein

Domains

• GP-PDE domain (aa 2-240) (according to UniProt)

Structure

• 2PZ0 (PDB) (GdpP from Thermoanaerobacter tengcongensis, 43% identity) PubMed
• P54527 (AlphaFold)

Paralogous protein(s)

• GlpQ, YhdW

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: yqiH-yqiI-yqiK

  Description: PubMed

  Regulation

  • only expressed under conditions of high osmolarity PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

• Genes: yqiK

  Description: PubMed

Biological materials

Mutant

• GP2384 ∆''yqiK::cat'', available in Jörg Stülke's lab
• MGNA-C458 (yqiK::erm), available at the NBRP B. subtilis, Japan
• BKE24180 (Δ''yqiK::ermC'') (available in the BGSC and in Jörg Stülke's lab) PubMed
• BKE24180 (ΔyqiK::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCGTTTCACCTCAAACCC,  downstream forward: _UP4_TAGTTGTTAGAAGGAGGCTG
• BKK24180 (ΔyqiK::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCGTTTCACCTCAAACCC,  downstream forward: _UP4_TAGTTGTTAGAAGGAGGCTG

References

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