PolY2

• Description: translesion synthesis (TLS-) DNA polymerase Y2
  
  

Gene name	polY2
Synonyms	yqjW
Essential	no
Product	translesion synthesis (TLS-) DNA polymerase Y2
Function	UV-targeted mutagenesis
Gene expression levels in SubtiExpress: polY2
Interactions involving this protein in SubtInteract: PolY2
MW, pI	45 kDa, 9.532
Gene length, protein length	1236 bp, 412 aa
Immediate neighbours	yqjX, yqzH
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

DNA repair/ recombination

This gene is a member of the following regulons

LexA regulon

The gene

Basic information

• Locus tag: BSU23710

Database entries

• BsubCyc: BSU23710

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1) (according to Swiss-Prot)

• Protein family: DNA polymerase type-Y family (according to Swiss-Prot)

• Paralogous protein(s): PolY1, UvrX, YozK-YobH

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • PolY2-DnaN (required for untargeted mutagenesis) PubMed
  • PolA-PolY2 PubMed

• Localization:

Database entries

• BsubCyc: BSU23710

• Structure:

• UniProt: P54560

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: polY2-yqjX (according to DBTBS)

• Expression browser: polY2 PubMed

• Sigma factor:

• Regulation:
  • induced upon DNA damage (LexA) PubMed

• Regulatory mechanism:
  • LexA: transcription repression PubMed

• Additional information:

Biological materials

• Mutant: GP1505 (polY2::kan), available in Stülke lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Justin S Lenhart, Jeremy W Schroeder, Brian W Walsh, Lyle A Simmons
DNA repair and genome maintenance in Bacillus subtilis.
Microbiol Mol Biol Rev: 2012, 76(3);530-64
[PubMed:22933559] [WorldCat.org] [DOI] (I p)