yqkA

168

unknown

Locus: BSU_23670

Molecular weight: 38.88 kDa

Isoelectric point: 8.16

Protein length: 343 aa

Gene length: 1032 bp

Function: unknown

Product: unknown

Essential: no

Synonyms: yqkA

Genomic Context

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List of homologs in different organisms, belongs to COG2320 (Galperin et al., 2021)

Gene

Coordinates: 2,462,193  2,463,224

The protein

Protein family

• C-terminal part: UPF0157 (GrpB) family (single member, according to UniProt)

Domains

• N-terminal N-acetyltransferase domain (according to UniProt)
• C-terminal GrpB superfamily domain

Structure

• 2NRK (PDB) (GrpB from Enterococcus faecalis, corresponds to the C-terminal domain, aa 153-318)
• P54564 (AlphaFold)

Expression and Regulation

Operons

• Genes: yqjY-yqjZ-yqkA-yqkB-yqkC

  Description: PubMed

• Genes: polY2-yqjX-yqjY-yqjZ-yqkA-yqkB-yqkC

  Description: PubMed

  Regulation

  • induced upon DNA damage (LexA) PubMed

  Regulatory mechanism

  • LexA: repression, PubMed, in lexA regulon

Biological materials

Mutant

• MGNA-C407 (yqkA::erm), available at the NBRP B. subtilis, Japan
• BKE23670 (yqkA::erm trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_ATTCAATCATTCGTGTTCTC,  downstream forward: _UP4_TTATGAACATACATGTGACA
• BKK23670 (yqkA::kan trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_ATTCAATCATTCGTGTTCTC,  downstream forward: _UP4_TTATGAACATACATGTGACA