spoVAB

168

essential for the uptake of the 1:1 chelate of pyridine-2,6-dicarboxylic acid (DPA(2,6)) and Ca(2+) into developing spores, required for spore maturation

Locus: BSU_23430

Molecular weight: 15.08 kDa

Isoelectric point: 10.12

Protein length: 141 aa

Gene length: 426 bp

Function: spore maturation

Product: unknown

Essential: no

E.C.: null

Synonyms: spoVAB

Genomic Context

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List of homologs in different organisms, belongs to COG5835 (Galperin et al., 2021)

Gene

Coordinates: 2,442,269 → 2,442,694

Phenotypes of a mutant

• the mutant initiates DPA release during germination faster than the wild type PubMed
• partially impaired localization of the SpoVAD "plug" in the spore inner membrane PubMed

The protein

Structure

• P40867 (AlphaFold)

Localization

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• Genes: spoVAA-spoVAB-spoVAC-spoVAD-spoVAEB-spoVAEA-spoVAF-lysA

  Description: PubMed

  Regulation

  • expressed late during sporulation in the forespore (SigG, SpoVT) PubMed

  Regulatory mechanism

  • SpoVT: activation, PubMed, in spoVT regulon

  Sigma factors

  • SigG: sigma factor, PubMed, in sigG regulon

Biological materials

Mutant

• BKE23430 (ΔspoVAB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CCCGACAAAAATGATGAACA,  downstream forward: _UP4_GACCATTCATAAGGAGGTTT
• BKK23430 (ΔspoVAB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CCCGACAAAAATGATGAACA,  downstream forward: _UP4_GACCATTCATAAGGAGGTTT

Labs working on this gene/protein

• Peter Setlow, University of Connecticut Health Center, USA

References

Reviews

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Original Publications

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