spmA

168

spore maturation protein (spore core dehydratation)

Locus: BSU_23180

Molecular weight: 21.35 kDa

Isoelectric point: 10.03

Protein length: 196 aa

Gene length: 591 bp

Function: germination

Product: spore maturation protein (spore core dehydratation)

Essential: no

E.C.: null

Synonyms: spmA, ypuJ

Genomic Context

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List of homologs in different organisms, belongs to COG2715 (Galperin et al., 2021)

Gene

Coordinates: 2,422,805 → 2,423,395

The protein

Structure

• P35157 (AlphaFold)

Localization

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• Genes: dacB-spmA-spmB

  Description: PubMed

  Regulation

  • expressed early during sporulation in the mother cell (SigE) PubMed

  Sigma factors

  • SigE: sigma factor, PubMed, in sigE regulon

Biological materials

Mutant

• MGNA-A002 (spmA::erm), available at the NBRP B. subtilis, Japan
• BKE23180 (ΔspmA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_AATTATATTGACCATTTTGC,  downstream forward: _UP4_CGCAAAAAGAAGGGAAGGTG
• BKK23180 (ΔspmA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_AATTATATTGACCATTTTGC,  downstream forward: _UP4_CGCAAAAAGAAGGGAAGGTG

References

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