Difference between revisions of "TrpE"

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(The trpE RNA switch)
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=== Database entries ===
 
=== Database entries ===
 +
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU22680&redirect=T BSU22680]
  
 
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/trpEDCFBA-hisC-tyrA-aroE.html]
 
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/trpEDCFBA-hisC-tyrA-aroE.html]
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=== Database entries ===
 
=== Database entries ===
 +
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU22680&redirect=T BSU22680]
  
 
* '''Structure:''' [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1I7Q 1I7Q] (from ''Serratia marcescens'', 42% identity, 62% similarity) {{PubMed|11371633}}
 
* '''Structure:''' [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1I7Q 1I7Q] (from ''Serratia marcescens'', 42% identity, 62% similarity) {{PubMed|11371633}}

Revision as of 14:03, 2 April 2014

  • Description: anthranilate synthase (subunit I)

Gene name trpE
Synonyms
Essential no
Product anthranilate synthase (subunit I)
Function biosynthesis of tryptophan
Gene expression levels in SubtiExpress: trpE
Interactions involving this protein in SubtInteract: TrpE
Metabolic function and regulation of this protein in SubtiPathways:
trpE
MW, pI 57 kDa, 5.246
Gene length, protein length 1545 bp, 515 aa
Immediate neighbours trpD, aroH
Sequences Protein DNA DNA_with_flanks
Genetic context
TrpE context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
TrpE expression.png















Categories containing this gene/protein

biosynthesis/ acquisition of amino acids

This gene is a member of the following regulons

TRAP regulon

The gene

Basic information

  • Locus tag: BSU22680

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate (according to Swiss-Prot)
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Modification:
  • Effectors of protein activity: subject to feedback inhibtion by tryptophan PubMed

Database entries

  • Structure: 1I7Q (from Serratia marcescens, 42% identity, 62% similarity) PubMed
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulatory mechanism:
    • TRAP: binding to the mRNA in the presence of tryptophan, this results in transcription termination PubMed
  • Additional information:
    • subject to feedback inhibtion by tryptophan PubMed
    • the mRNA is substantially stabilized upon depletion of RNase Y PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

The trpE RNA switch


Other original publications