lhaT

168

lipoprotein heptaprenylglyceryl N-acetyl transferase, required for the N-acetylation of lipoproteins

Locus: BSU_22530

Molecular weight: 21.00 kDa

Isoelectric point: 5.354

Protein length: 185 aa

Gene length: 558 bp

Function: N-acetylation of lipoproteins

Product: lipoprotein heptaprenylglyceryl N-acetyl transferase

Essential: no

Synonyms: ypjA

Genomic Context

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List of homologs in different organisms, belongs to COG4347 (Galperin et al., 2021)

Gene

Coordinates: 2,362,407 → 2,362,964

Phenotypes of a mutant

• lack of lipoprotein N-acetylation PubMed

The protein

Catalyzed reaction/ biological activity

• Ac-HepG + α-amino cysteine group of diacylglyceryl lipoproteins --> acetylated lipoproteins (the reaction takes place at the outer side of the membrane) PubMed

Structure

• P54392 (AlphaFold)

Localization

• integral membrane protein PubMed

Biological materials

Mutant

• MGNA-A424 (ypjA::erm), available at the NBRP B. subtilis, Japan
• BKE22530 (ΔlhaT::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCGGCCGCTGCCCTAATA,  downstream forward: _UP4_TAAAGACAATGTTGGTCTAT
• BKK22530 (ΔlhaT::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCGGCCGCTGCCCTAATA,  downstream forward: _UP4_TAAAGACAATGTTGGTCTAT

References

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