pbuX

168

xanthine transport in/out via proton symport

Locus: BSU_22060

Molecular weight: 46.08 kDa

Isoelectric point: 8.79

Protein length: 438 aa

Gene length: 1317 bp

Function: xanthine uptake

Product: xanthine permease

Essential: no

Synonyms: pbuX, ypaQ

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG2233 (Galperin et al., 2021)

Gene

Coordinates: 2,318,127  2,319,443

The protein

Protein family

• xanthine/uracil permease family (according to UniProt)
• Nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) subfamily (according to UniProt)

Structure

• 5I6C (PDB) (Aspergillus nidulans purine transporter, 27% identity) PubMed
• P42086 (AlphaFold)

Paralogous protein(s)

• PucK, PucJ

Localization

• membrane PubMed

Expression and Regulation

Operons

• Genes: xpt-pbuX

  Description: PubMed

  Regulation

  • induced in the absence of guanine (G-box) PubMed

  Regulatory mechanism

  • G-box: RNA switch, riboswitch PubMed, in G-box
  • PurR: repression, (molecular inducer: PRPP) PubMed, in purR regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• BKE22060 (pbuX::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAGCGTTTTGCCGAATCCAT,  downstream forward: _UP4_AAAACAGCAGTCTAACTCCG
• BKK22060 (pbuX::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAGCGTTTTGCCGAATCCAT,  downstream forward: _UP4_AAAACAGCAGTCTAACTCCG

References

Loading