blyA

168

N-acetylmuramoyl-L-alanine amidase

Locus: BSU_21410

Molecular weight: 39.47 kDa

Isoelectric point: 9.94

Protein length: 367 aa

Gene length: 1104 bp

Function: SPß-mediated lysis

Product: N-acetylmuramoyl-L-alanine amidase

Essential: no

E.C.: 3.5.1.28

Synonyms: blyA, yomC

Genomic Context

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List of homologs in different organisms, belongs to COG5632 (Galperin et al., 2021)

Gene

Coordinates: 2,263,489 → 2,264,592

The protein

Catalyzed reaction/ biological activity

• Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides (according to UniProt)

Protein family

• N-acetylmuramoyl-L-alanine amidase 2 family (according to UniProt)

Domains

• contains an amidase_2 domain (like CwlA, CwlH, XlyA, XlyB)
• 2 SH3B domains 1 (aa 202-271, aa 298-367) (according to UniProt)
• N-acetylmuramoyl-L-alanine amidase domain (aa 24-158) (according to UniProt)

Structure

• O31982 (AlphaFold)

Localization

• secreted (according to UniProt)

Biological materials

Mutant

• BKE21410 (ΔblyA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAAAAACATCTCCTTAAT,  downstream forward: _UP4_TAATTATATTCAATTTTCTC
• BKK21410 (ΔblyA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAAAAACATCTCCTTAAT,  downstream forward: _UP4_TAATTATATTCAATTTTCTC

References

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