yonN

168

SP prophage-derived DNA-binding protein HU 2

Locus: BSU_21050

Molecular weight: 9.71 kDa

Isoelectric point: 10.23

Protein length: 92 aa

Gene length: 279 bp

Function: untwisting of the DNA double helix

Product: DNA-binding protein HU 2

Essential: no

Synonyms: yonN

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0776 (Galperin et al., 2021)

Gene

Coordinates: 2,225,337  2,225,615

The protein

Catalyzed reaction/ biological activity

• YonN-mediated untwisting of the DNA stimulates Nth endonuclease activity at AP sites PubMed

Protein family

• bacterial histone-like protein family (with Hbs, according to UniProt)

Structure

• 1HUE (PDB) (Hbs from B. stearothermophilus, 71% identity) PubMed
• P68573 (AlphaFold)

Paralogous protein(s)

• Hbs

Expression and Regulation

Operons

• Genes: yonP-yonO-yonN

  Description: PubMed

Biological materials

Mutant

• BKE21050 (yonN::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAATCAATTTCCTCCTAA,  downstream forward: _UP4_TAATCAGTTAATGAGGACGA
• BKK21050 (yonN::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAATCAATTTCCTCCTAA,  downstream forward: _UP4_TAATCAGTTAATGAGGACGA

References

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