yonO

168

SP beta DNA-dependent RNA polymerase

Locus: BSU_21040

Molecular weight: 97.78 kDa

Isoelectric point: 9.06

Protein length: 839 aa

Gene length: 2520 bp

Function: transcription of late SP beta genes

Product: SP beta RNA polymerase

Essential: no

Synonyms: yonO

Genomic Context

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List of homologs in different organisms

Gene

Coordinates: 2,222,574  2,225,093

The protein

Catalyzed reaction/ biological activity

• ribonucleoside 5'-triphosphate + RNA_(n) --> diphosphate + RNA_(n+1) (according to UniProt)

Protein family

• YRH RNA polymerase family (single member, according to UniProt)

Structure

• O31945 (AlphaFold)

Expression and Regulation

Operons

• Genes: yonP-yonO-yonN

  Description: PubMed

Biological materials

Mutant

• BKE21040 (yonO::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAATAAATTCTCCCCTTATT,  downstream forward: _UP4_TAGAAAGTGCCTTGAGCCTT
• BKK21040 (yonO::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAATAAATTCTCCCCTTATT,  downstream forward: _UP4_TAGAAAGTGCCTTGAGCCTT

References

Reviews

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Research papers

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