aimP

168

peptide, part of the arbitrium peptide-based communication system, processed AimP peptide inactivates AimR

Locus: BSU_20850

Molecular weight: 4.08 kDa

Isoelectric point: 6.26

Protein length: 41 aa

Gene length: 126 bp

Function: control of AimR activity

Product: arbitrium peptide

Essential: no

E.C.: null

Synonyms: aimP, AimP, yopL

Genomic Context

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List of homologs in different organisms

Gene

Coordinates: 2,208,855  2,208,980

Phenotypes of a mutant

• increased prophage production PubMed
• sharper plaque morphology (similar to yopN mutant) PubMed

The protein

Catalyzed reaction/ biological activity

• Proteolytically cleaved in extracellular space to yield hexapeptide (GMPRGA) regulator. Hexapeptide re-enters cells and binds to AimR to promote SPbeta lysogeny.  (PubMed)

Structure

• Hexapeptide bound to AimR: 5Y24 (PDB) (PubMed), 5ZW6 (PDB) (PubMed), 6IM4 (PDB) (PubMed), 6HP5 (PDB) (PubMed), 6JG9 (PDB) (PubMed)
• O31926 (AlphaFold)

Expression and Regulation

Operons

• Genes: aimR-aimP

  Description

  • PubMed

  Expression

  • repressed by MrpR

  Regulatory mechanism

  • MrpR: repression, in mrpR regulon

• Genes: aimP

  Description: PubMed

  Regulation

  • expressed during the lytic cycle PubMed

  Regulatory mechanism

  • CcpA: repression, CcpA: transcription repression PubMed, in ccpA regulon

Additional information

• produce as 43 aa pro-peptide, secreted to the medium, and processed to the mature 6 aa AimX peptide that can be taken up by the cells again PubMed

Biological materials

Mutant

• BKE20850 (aimP::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTGTCTCACCTCCTTTAA,  downstream forward: _UP4_TAAAATCCATTGACACATAA
• BKK20850 (aimP::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTGTCTCACCTCCTTTAA,  downstream forward: _UP4_TAAAATCCATTGACACATAA

References

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