yojE

168

unknown

Locus: BSU_19480

Molecular weight: 33.23 kDa

Isoelectric point: 10.01

Protein length: 298 aa

Gene length: 897 bp

Function: unknown

Product: unknown

Essential: no

E.C.: null

Synonyms: yojE

Genomic Context

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List of homologs in different organisms, belongs to COG2962 (Galperin et al., 2021)

Gene

Coordinates: 2,123,026 → 2,123,922

The protein

Protein family

• EamA transporter family (according to UniProt)

Domains

• 2 EamA domains (aa 17-148, aa 162-286) (according to UniProt)

Structure

• O31859 (AlphaFold)

Expression and Regulation

Operons

• Genes: yojE-yoyC-yojF-bshB2

  Description: PubMed

  Regulation

  • ''yoyC'': induced by diamide stess (thiol depletion) (Spx) PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• MGNA-B430 (yojE::erm), available at the NBRP B. subtilis, Japan
• BKE19480 (ΔyojE::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTGATATCCCTGCCTTTC,  downstream forward: _UP4_TGAGGCCTTTGGCTTTTCAG
• BKK19480 (ΔyojE::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTGATATCCCTGCCTTTC,  downstream forward: _UP4_TGAGGCCTTTGGCTTTTCAG

Expression system

• GP4621 (trpC2 xkdE::P_xyl-yojE-ermC), available in Jörg Stülke's lab

References

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