des

168

phospholipid desaturase

Locus: BSU_19180

Molecular weight: 40.55 kDa

Isoelectric point: 9.88

Protein length: 352 aa

Gene length: 1059 bp

Function: adaptation of membrane fluidity at low temperatures

Product: phospholipid desaturase

Essential: no

Synonyms: des, yocE

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG3239 (Galperin et al., 2021)

Gene

Coordinates: 2,089,396  2,090,454

Phenotypes of a mutant

• cold-sensitive PubMed
• a strain lacking the ptb-bcd-buk-lpdV-bkdAA-bkdAB-bkdB operon and the des gene exhibits strongly reduced membrane fluidity and eventual growth arrest PubMed

The protein

Catalyzed reaction/ biological activity

• introduces double bonds into long chain fatty acids PubMed

Protein family

• Fatty acid desaturase type 1 family (single member, according to UniProt)

Cofactors

• Des receives electrons from ferredoxin (Fer) or from either of the two flavodoxins, YkuN or YkuP, the three proteins can functionally replace each other PubMed

Structure

• O34653 (AlphaFold)

Localization

• integral membrane protein PubMed

Expression and Regulation

Operons

• Genes: des

  Description: PubMed

  Regulation

  • induced upon cold shock (DesR) PubMed

  Regulatory mechanism

  • DesR: activation, PubMed, PubMed, in desR regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

  Additional information

  • in strains evolved for growth at low pressure, 'des' expression is significantly increased as compared to the wild type PubMed

Biological materials

Mutant

• MGNA-A319 (yocE::erm), available at the NBRP B. subtilis, Japan
• 1A835 ( des::kan), PubMed, available at BGSC
• BKE19180 (des::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAGTAAGTATCCTCTCAT,  downstream forward: _UP4_TGATGATAAAGGAGGTCTTC
• BKK19180 (des::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAGTAAGTATCCTCTCAT,  downstream forward: _UP4_TGATGATAAAGGAGGTCTTC

Labs working on this gene/protein

• Diego de Mendoza, Universidad Nacional de Rosario, Argentine homepage

References

Reviews

Loading

Original Publications

Loading