pps

168

similar to rifampicin phosphotransferase

Locus: BSU_18830

Molecular weight: 97.00 kDa

Isoelectric point: 5.29

Protein length: 866 aa

Gene length: 2601 bp

Function: resistance to rifampicin

Product: similar to rifampicin phosphotransferase

Essential: no

Synonyms: pps

Genomic Context

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List of homologs in different organisms, belongs to COG0574 (Galperin et al., 2021)

Gene

Coordinates: 2,051,329 → 2,053,929

The protein

Protein family

• PEP-utilizing enzyme family (according to UniProt)

Structure

• 5HV1 (PDB) (from Listeria monocytogenes, 62% identity) PubMed
• O34309 (AlphaFold)

Paralogous protein(s)

• YvkC

Additional information

• The gene is annotated in KEGG and MetaCyc as phosphoenolpyruvate synthase EC 2.7.9.2. In Swiss-Prot the protein is described as “phosphoenolpyruvate synthase”. No literature/experimental evidence supporting the annotation is available.  PubMed

Expression and Regulation

Operons

• Genes: pps

  Description: PubMed

Biological materials

Mutant

• BKE18830 (Δpps::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTCTACATCTCCCCTTCA,  downstream forward: _UP4_TAATGTCTGATGTGTGTAGA
• BKK18830 (Δpps::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTCTACATCTCCCCTTCA,  downstream forward: _UP4_TAATGTCTGATGTGTGTAGA

References

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