yoaV

168

putative cysteine and O-acetyl serine efflux permease

Locus: BSU_18770

Molecular weight: 32.89 kDa

Isoelectric point: 9.43

Protein length: 292 aa

Gene length: 879 bp

Function: unknown

Product: putative cysteine and O-acetyl serine efflux permease

Essential: no

E.C.: null

Synonyms: yoaV, cyeA

Genomic Context

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List of homologs in different organisms, belongs to COG0697 (Galperin et al., 2021)

Gene

Coordinates: 2,045,929  2,046,807

The protein

Protein family

• EamA transporter family (according to UniProt)

Domains

• 2 EamA domains (aa 13-137, aa 159-285) (according to UniProt)

Structure

• O34416 (AlphaFold)

Expression and Regulation

Operons

• Genes: yoaS-yozG-yoaT-yoaV

  Description: PubMed

Biological materials

Mutant

• GP4809 (yoaV::neo), available in Jörg Stülke's lab
• MGNA-B401 (yoaV::erm), available at the NBRP B. subtilis, Japan
• BKE18770 (yoaV::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAATGAAGCCGCCTTTCT,  downstream forward: _UP4_TGAATGATATACATTTCCCC
• BKK18770 (yoaV::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAATGAAGCCGCCTTTCT,  downstream forward: _UP4_TGAATGATATACATTTCCCC

Expression system

• GP4620 (trpC2 xkdE::P_xyl-yoaV-ermC), available in Jörg Stülke's lab