yoaD

yoaD
168

similar to phosphoglycerate dehydrogenase

Locus
BSU_18560
Molecular weight
38.47 kDa
Isoelectric point
5.62
Protein length
Gene length
Function
methionine biosynthesis
Product
unknown
Essential
no
Synonyms
yoaD

Genomic Context

List of homologs in different organisms, belongs to COG0111 (Galperin et al., 2021)

This gene is a member of the following regulons

S-box

Gene
Coordinates
2,024,042  2,025,076
The protein
Catalyzed reaction/ biological activity
YoaD is likely to convert 3-phosphoglycerate to serine for use in methionine biosynthesis (based on S-box regulation)
Protein family
D-isomer specific 2-hydroxyacid dehydrogenase family (with YvcT and SerA, according to UniProt)
Structure
1WWK (PDB) (phosphoglycerate dehydrogenase from Pyrococcus horikoshii, 35% identity)
Additional information
The gene is annotated in KEGG as an ortholog of D-3-phosphoglycerate dehydrogenase EC 1.1.1.95. No EC annotation is available in Swiss-Prot. In MetaCyc the protein is marked as similar to phosphorglycerate dehydrogenase. No literature/experimental evidence supporting the annotation is available.  PubMed
Expression and Regulation
Operons
Description
Regulation
induced by methionine starvation (S-box) PubMed
the S-box RNA is degraded by RNase Y PubMed
Regulatory mechanism
S-box: RNA switch, , the S-box riboswitch binds S-adenosylmethionine resulting in termination PubMed, in S-box
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yoaDyoaB

2025-07-24 11:53:30

Jstuelk

114

0b52fbe0bb9d5809477459667c1400f3004ff193

D97B471EE690CF8F0B0DBE37D2272E2DA13A7F83

Biological materials
Mutant
MGNA-A835 (yoaD::erm), available at the NBRP B. subtilis, Japan
BKE18560 (yoaD::erm  trpC2) available at BGSCPubMed, upstream reverse: _UP1_CATAAAAAAAATCTCTTTCC,  downstream forward: _UP4_TAAGAAAGGAGGCTAACAGA
BKK18560 (yoaD::kan  trpC2) available at BGSCPubMed, upstream reverse: _UP1_CATAAAAAAAATCTCTTTCC,  downstream forward: _UP4_TAAGAAAGGAGGCTAACAGA
References
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Page visits: 4371

Time of last update: 2025-07-25 22:42:06

Author of last update: Melvin.boenninger