yofA

168

transcriptional activator of ftsW at the onset of stationary phase

Locus: BSU_18420

Molecular weight: 31.90 kDa

Isoelectric point: 6.16

Protein length: 285 aa

Gene length: 858 bp

Function: control of ftsW expression

Product: transcriptional activator (LysR family)

Essential: no

Synonyms: yofA, ftsR

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0583 (Galperin et al., 2021)

Gene

Coordinates: 2,006,541  2,007,398

The protein

Protein family

• LysR family (according to UniProt)

Domains

• HTH lysR-type domain (aa 1-58) (according to UniProt)

Structure

• 4X6G (PDB) (from Pseudomonas aeruginosa, 25% identity) PubMed
• O34685 (AlphaFold)

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: yofA

  Description: PubMed

  Regulation

  • maximally expressed at the onset of stationary phase PubMed

Biological materials

Mutant

• MGNA-B090 (yofA::erm), available at the NBRP B. subtilis, Japan
• BKE18420 (yofA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACGCGCTCTCACCTGCTTG,  downstream forward: _UP4_TGAAGATAGACGTGCATTAG
• BKK18420 (yofA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACGCGCTCTCACCTGCTTG,  downstream forward: _UP4_TGAAGATAGACGTGCATTAG

References

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