yofA

168

transcriptional activator of [gene|8AB7D225DBEE6BD695A4A8A2384D2C029B571C57|ftsW] at the onset of stationary phase

locus: BSU_18420

Molecular weight: 31.90 kDa

pI: 6.16

Protein length: 285 aa

Gene length: 858 bp

function: control of [gene|8AB7D225DBEE6BD695A4A8A2384D2C029B571C57|ftsW] expression

product: transcriptional activator ([wiki|LysR family])

essential: no

synonyms: yofA, ftsR

List of homologs in different organisms, belongs to COG0583 (Galperin et al., 2021)

Gene

Coordinates: 2,006,541  2,007,398

The protein

Protein family

• [wiki|LysR family] (according to UniProt)

[wiki|Domains]

• [wiki|HTH lysR-type domain] (aa 1-58) (according to UniProt)

Structure

• [PDB|4X6G] (from Pseudomonas aeruginosa, 25% identity) [pubmed|25931525]
• [AF|O34685]

[wiki|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|6FDA046DCBBAE4588C4BC7163A04B9E6AEDBAC55|yofA]

  description: [Pubmed|22383849]

  regulation

  • maximally expressed at the onset of stationary phase [Pubmed|17526699]

Biological materials

Mutant

• MGNA-B090 (yofA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1089 NBRP B. subtilis, Japan]
• BKE18420 ([gene|6FDA046DCBBAE4588C4BC7163A04B9E6AEDBAC55|yofA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE18420 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACGCGCTCTCACCTGCTTG,  downstream forward: _UP4_TGAAGATAGACGTGCATTAG
• BKK18420 ([gene|6FDA046DCBBAE4588C4BC7163A04B9E6AEDBAC55|yofA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK18420 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACGCGCTCTCACCTGCTTG,  downstream forward: _UP4_TGAAGATAGACGTGCATTAG

References

• 17526699,22383849,25931525