ppsC

168

plipastatin synthetase

Locus: BSU_18320

Molecular weight: 287.19 kDa

Isoelectric point: 5.35

Protein length: 2555 aa

Gene length: 7668 bp

Function: production of the antibacterial compound plipastatin

Product: plipastatin synthetase

Essential: no

Synonyms: ppsC

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG4908 (Galperin et al., 2021)

Gene

Coordinates: 1,974,881 → 1,982,548

The protein

Protein family

• ATP-dependent AMP-binding enzyme family (according to UniProt)

Domains

• 2 Carrier domains (aa 967-1042, aa 2003-2077) (according to UniProt)

Structure

• 2VSQ (PDB) (termination module of SrfAC, 37% identity) PubMed
• P39847 (AlphaFold)

Expression and Regulation

Operons

• Genes: ppsA-ppsB-ppsC-ppsD-ppsE

  Description: PubMed

  Regulatory mechanism

  • AbrB: repression, PubMed, in abrB regulon

Biological materials

Mutant

• BKE18320 (ΔppsC::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CTGCGGCATCAGTGAATCTC,  downstream forward: _UP4_TAGAAAATCGTGACAGGAGA
• BKK18320 (ΔppsC::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CTGCGGCATCAGTGAATCTC,  downstream forward: _UP4_TAGAAAATCGTGACAGGAGA

References

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