uptA

168

undecaprenyl phosphate transporter A, regeneration of the cytoplasmic pool of undecaprenyl phosphate for peptidoglycan and teichoic acid biosynthesis

Locus: BSU_18190

Molecular weight: 21.78 kDa

Isoelectric point: 9.77

Protein length: 198 aa

Gene length: 597 bp

Function: recycling of undecaprenyl phosphate for peptidoglycan and teichoic acid biosynthesis

Product: undecaprenyl phosphate transporter A

Essential: no

Synonyms: yngC

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0586 (Galperin et al., 2021)

Gene

Coordinates: 1,947,668  1,948,264

The protein

Catalyzed reaction/ biological activity

• flips surface-exposed undecaprenyl phosphate to the cytoplasmic side PubMed

Protein family

• dedA family (with PetA and YkoX, according to UniProt) PubMed

Structure

• O31823 (AlphaFold)

Paralogous protein(s)

• PetA
• YkoX, (43%)

Localization

• cell membrane PubMed

Expression and Regulation

Operons

• Genes: yngA-yngB-uptA

  Description: PubMed

  Regulation

  • expression during spore germination is strongly reduced under conditions of osmotic stress PubMed
  • expressed under anaerobic conditions (YclJ) PubMed

  Regulatory mechanism

  • YclJ: activation, PubMed, in yclJ regulon

• Genes: uptA

  Description: PubMed

  Sigma factors

  • SigM: sigma factor, PubMed, in sigM regulon

Biological materials

Mutant

• MGNA-B397 (yngC::erm), available at the NBRP B. subtilis, Japan
• BKE18190 (uptA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACATTCTTCACAACCTGTC,  downstream forward: _UP4_TAGCTAGCGGATATGCATAG
• BKK18190 (uptA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACATTCTTCACAACCTGTC,  downstream forward: _UP4_TAGCTAGCGGATATGCATAG

Reviews

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References

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