yncD

168

spore coat alanine racemase

Locus: BSU_17640

Molecular weight: 43.49 kDa

Isoelectric point: 9.59

Protein length: 394 aa

Gene length: 1182 bp

Function: protection of the spore

Product: spore coat alanine racemase

Essential: no

E.C.: 5.1.1.1

Synonyms: yncD, alrB

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0787 (Galperin et al., 2021)

Gene

Coordinates: 1,897,941 → 1,899,125

The protein

Catalyzed reaction/ biological activity

• L-alanine --> D-alanine (according to UniProt)

Protein family

• alanine racemase family (with Alr, according to UniProt)

Cofactors

• PLP (according to UniProt)

Structure

• 5IRP (PDB) (from Bacillus subtilis 100% identity)
• 6Q70 (PDB) (crystallized with HEPES) PubMed
• 6Q71 (PDB) (crystallized with Bis-Tris Propane) PubMed
• 6Q72 (PDB) PubMed
• P94494 (AlphaFold)

Paralogous protein(s)

• Alr

Localization

• outer spore coat, localization depends on CotE PubMed

Expression and Regulation

Operons

• Genes: yncD

  Description: PubMed

  Regulation

  • expressed during sporulation in the mother cell (SigE) PubMed

  Sigma factors

  • SigE: sigma factor, PubMed, in sigE regulon

Biological materials

Mutant

• MGNA-B099 (yncD::erm), available at the NBRP B. subtilis, Japan
• BKE17640 (ΔyncD::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCAACGCTTCCTTCCTTC,  downstream forward: _UP4_TAGAACATCTTAAAATGCTG
• BKK17640 (ΔyncD::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCAACGCTTCCTTCCTTC,  downstream forward: _UP4_TAGAACATCTTAAAATGCTG

References

Reviews

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Original Publications

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