yncB

168

similar to micrococcal nuclease

Locus: BSU_17620

Molecular weight: 23.54 kDa

Isoelectric point: 9.21

Protein length: 211 aa

Gene length: 636 bp

Function: unknown

Product: unknown

Essential: no

Synonyms: yncB

Genomic Context

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List of homologs in different organisms, belongs to COG1525 (Galperin et al., 2021)

Gene

Coordinates: 1,895,378  1,896,013

The protein

Protein family

• thermonuclease family (single member, according to UniProt)

Structure

• 4I65 (PDB) (from Staphylococcus aureus, corresponds to aa 69 ... 169 of YncB, 58% identity)
• P94492 (AlphaFold)

Paralogous protein(s)

• YokF

Localization

• Cytoplasm (homogeneous) PubMed

Expression and Regulation

Operons

• Genes: yncB

  Description: PubMed

Biological materials

Mutant

• MGNA-B100 (yncB::erm), available at the NBRP B. subtilis, Japan
• BKE17620 (yncB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATTGATTCCCTCTTTAT,  downstream forward: _UP4_TAGAAAAACACTGTTTGTTA
• BKK17620 (yncB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATTGATTCCCTCTTTAT,  downstream forward: _UP4_TAGAAAAACACTGTTTGTTA

References

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