miaA

168

tRNA isopentenylpyrophosphate transferase

Locus: BSU_17330

Molecular weight: 35.52 kDa

Isoelectric point: 6

Protein length: 314 aa

Gene length: 945 bp

Function: tRNA modification

Product: tRNA isopentenylpyrophosphate transferase

Essential: no

E.C.: 2.5.1.8

Synonyms: miaA

Genomic Context

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Categories containing this gene/protein

Information processing, Protein synthesis, modification and degradation, Translation, tRNA modification and maturation

List of homologs in different organisms, belongs to COG0324 (Galperin et al., 2021)

Gene

Coordinates: 1,866,389  1,867,333

Phenotypes of a mutant

inactivation of ''miaA'' reduces sporulation efficiency to 16% that of wild type cells PubMed

The protein

Catalyzed reaction/ biological activity

adenosine³⁷ in tRNA + dimethylallyl diphosphate --> diphosphate + N⁶-dimethylallyladenosine³⁷ in tRNA (according to UniProt)

Protein family

IPP transferase family (single member, according to UniProt)

Structure

2QGN (PDB) (from Bacillus halodurans, 57% identity)
O31795 (AlphaFold)

Expression and Regulation

Operons

Genes: miaA-hfq

Description: PubMed

Genes: gerY-miaA-hfq

Description: PubMed

Regulation

expressed during sporulation in the mother cell (SigE) PubMed

Sigma factors

SigE: sigma factor, PubMed, in sigE regulon

Biological materials

Mutant

BKE17330 (miaA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAATATGTATCACTCTCTTT,  downstream forward: _UP4_TAATCGAAACTGTATGATAT
BKK17330 (miaA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAATATGTATCACTCTCTTT,  downstream forward: _UP4_TAATCGAAACTGTATGATAT

References

The corresponding gene/ protein in other bacteria

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