miaA

168

tRNA isopentenylpyrophosphate transferase

locus: BSU_17330

Molecular weight: 35.52 kDa

pI: 6

Protein length: 314 aa

Gene length: 945 bp

function: tRNA modification

product: tRNA isopentenylpyrophosphate transferase

essential: no

ec: 2.5.1.8

synonyms: miaA

List of homologs in different organisms, belongs to COG0324 (Galperin et al., 2021)

Gene

Coordinates: 1,866,389  1,867,333

Phenotypes of a mutant

• inactivation of ''[gene|09C9CDA3EA23B751C7135DEDF0FE2563BA69FF1D|miaA]'' reduces sporulation efficiency to 16% that of wild type cells [Pubmed|26735940]

The protein

Catalyzed reaction/ biological activity

• adenosine³⁷ in tRNA + dimethylallyl diphosphate --> diphosphate + N⁶-dimethylallyladenosine³⁷ in tRNA (according to UniProt)

Protein family

• IPP transferase family (single member, according to UniProt)

Structure

• [PDB|2QGN] (from ''Bacillus halodurans'', 57% identity)
• [AF|O31795]

Expression and Regulation

Operons

• genes: [gene|09C9CDA3EA23B751C7135DEDF0FE2563BA69FF1D|miaA]-[gene|40D3FE1A512703B090316C31099956DB6A7F515B|hfq]

  description: [pubmed|22383849]

• genes: [gene|61B511C49912F1380AEE18AD300255A435F56F94|gerY]-[gene|09C9CDA3EA23B751C7135DEDF0FE2563BA69FF1D|miaA]-[gene|40D3FE1A512703B090316C31099956DB6A7F515B|hfq]

  description: [pubmed|22383849]

  regulation

  • expressed during [wiki|sporulation ] in the mother cell ([protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]) [Pubmed|39530705,22383849]

  sigma factors

  • [protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]: sigma factor, [pubmed|39530705], in [regulon|protein:3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE regulon]

Biological materials

Mutant

• BKE17330 ([gene|09C9CDA3EA23B751C7135DEDF0FE2563BA69FF1D|miaA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE17330 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAATATGTATCACTCTCTTT,  downstream forward: _UP4_TAATCGAAACTGTATGATAT
• BKK17330 ([gene|09C9CDA3EA23B751C7135DEDF0FE2563BA69FF1D|miaA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK17330 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAATATGTATCACTCTCTTT,  downstream forward: _UP4_TAATCGAAACTGTATGATAT

References

The corresponding gene/ protein in other bacteria

• 11160115,3045085,9294434,26735940,35212379