aprX

168

intracellular alkaline serine protease

Locus: BSU_17260

Molecular weight: 47.74 kDa

Isoelectric point: 4.97

Protein length: 442 aa

Gene length: 1329 bp

Function: protein degradation

Product: intracellular alkaline serine protease

Essential: no

Synonyms: aprX

Genomic Context

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Categories containing this gene/protein

Metabolism, Amino acid/ nitrogen metabolism, Utilization of nitrogen sources other than amino acids, Utilization of proteins

List of homologs in different organisms, belongs to COG1404 (Galperin et al., 2021)

Gene

Coordinates: 1,861,384  1,862,712

The protein

Protein family

peptidase S8 family (according to UniProt)

Domains

Peptidase S8 domain (aa 122-439) (according to UniProt)

Structure

3WHI (PDB) (AprE, corresponds to aa 78 ... 436, 33% identity) PubMed
O31788 (AlphaFold)

Expression and Regulation

Operons

Genes: aprX

Description: PubMed

Regulation

induced by DNA damage (LexA) PubMed

Regulatory mechanism

LexA: repression, PubMed, in lexA regulon

Sigma factors

SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

MGNA-A019 (aprX::erm), available at the NBRP B. subtilis, Japan
BKE17260 (aprX::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTATTTTCCTCCTATTA,  downstream forward: _UP4_TAAACATCATCAAAAGCCGG
BKK17260 (aprX::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTATTTTCCTCCTATTA,  downstream forward: _UP4_TAAACATCATCAAAAGCCGG

References

Reviews

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Original Publications

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Labs working on this gene/protein

Alessandra Albertini, University of Pavia, Italy homepage