pksH

168

involved in polyketide synthesis

Locus: BSU_17160

Molecular weight: 29.26 kDa

Isoelectric point: 6.33

Protein length: 259 aa

Gene length: 780 bp

Function: polyketide synthesis

Product: unknown

Essential: no

E.C.: null

Synonyms: pksH

Genomic Context

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List of homologs in different organisms, belongs to COG1024 (Galperin et al., 2021)

Gene

Coordinates: 1,791,193  1,791,972

The protein

Protein family

• enoyl-CoA hydratase/isomerase family (according to UniProt)

Structure

• 3HP0 (PDB)
• P40805 (AlphaFold)

Expression and Regulation

Operons

• Genes: pksC-pksD-pksE-acpK-pksF-pksG-pksH-pksI-pksJ-pksL-pksM-pksN-pksR

  Description: PubMed

  Regulation

  • expressed during the transition from growth to stationary phase (AbrB, CodY) PubMed
  • heterogeneous expression, percentage of expressing cells increases during colonization of plant roots PubMed

  Regulatory mechanism

  • AbrB: repression, PubMed, in abrB regulon
  • CodY: activation, PubMed, in codY regulon

  Additional information

  • this is a very large operon comprising about 75 kb

• Genes: pksB-pksC-pksD-pksE-acpK-pksF-pksG-pksH-pksI-pksJ-pksL-pksM-pksN-pksR

  Description: PubMed

Biological materials

Mutant

• BKE17160 (pksH::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GCGGACCTTTATCGTTTGAT,  downstream forward: _UP4_TAACCGTTTAAAAATGACAA
• BKK17160 (pksH::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GCGGACCTTTATCGTTTGAT,  downstream forward: _UP4_TAACCGTTTAAAAATGACAA

References

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