CotE

• Description: outer spore coat morphogenetic protein, controls the assembly of the outer spore coat layer
  
  

Gene name	cotE
Synonyms
Essential	no
Product	spore coat morphogenetic protein
Function	assembly of the outer spore coat
Gene expression levels in SubtiExpress: cotE
Interactions involving this protein in SubtInteract: CotE
MW, pI	20 kDa, 4.139
Gene length, protein length	543 bp, 181 aa
Immediate neighbours	ymcA, mutS
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

sporulation proteins

This gene is a member of the following regulons

SigE regulon, SigK regulon, GerE regulon

CotE-dependent proteins of the outer spore coat

• CotA, CotB, CotG, CotM, CotO, CotQ, CotS, CotU, CotW, CotZ, Tgl, YknT, YlbD, YncD, YtxO PubMed

The gene

Basic information

• Locus tag: BSU17030

Phenotypes of a mutant

• mis-assembly of the outer spore coat PubMed

Database entries

• BsubCyc: BSU17030

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • assembly of the CotC-CotU complex PubMed

• Protein family: cotE family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:
  • homotypic interaction PubMed
  • CotE-CotC PubMed
  • CotE-CotU PubMed
  • SpoVID-CotE PubMed

• Localization:
  • encircles the forespore with a biased accumulation on the mother cell-proximal face of the forespore PubMed
  • outer spore coat, localization depends on SpoIVA PubMed

Database entries

• BsubCyc: BSU17030

• Structure:

• UniProt: P14016

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: cotE PubMed

• Expression browser: cotE PubMed

• Sigma factor: SigE PubMed, SigK PubMed

• Regulation:
  • repressed by casamino acids PubMed
  • expressed during sporulation in the mother cell (SigE, SigK, GerE) PubMed

• Regulatory mechanism:
  • GerE: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:
  • 1S105 ( cotE::cat), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Arthur Aronson, Purdue University, West Lafayette, USA homepage

Adriano Henriques, Lisbon, Portugal homepage

References

Reviews

Peter T McKenney, Adam Driks, Patrick Eichenberger
The Bacillus subtilis endospore: assembly and functions of the multilayered coat.
Nat Rev Microbiol: 2013, 11(1);33-44
[PubMed:23202530] [WorldCat.org] [DOI] (I p)

Peter Setlow
Dynamics of the assembly of a complex macromolecular structure--the coat of spores of the bacterium Bacillus subtilis.
Mol Microbiol: 2012, 83(2);241-4
[PubMed:22192522] [WorldCat.org] [DOI] (I p)