spoVFB

168

dipicolinate synthase (subunit B)

Locus: BSU_16740

Molecular weight: 21.72 kDa

Isoelectric point: 6.15

Protein length: 200 aa

Gene length: 603 bp

Function: dipicolic acid production

Product: dipicolinate synthase (subunit B)

Essential: no

Synonyms: spoVFB, dpaB

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG5843 (Galperin et al., 2021)

Gene

Coordinates: 1,745,263  1,745,865

The protein

Catalyzed reaction/ biological activity

• (S)-2,3-dihydrodipicolinate + NADP⁺ --> dipicolinate + H⁺ + NADPH (according to UniProt)

Structure

• 3MCU (PDB) (from B. cereus, 72% identity)
• Q04810 (AlphaFold)

Expression and Regulation

Operons

• Genes: spoVFA-spoVFB-asd-dapG-dapA

  Description: PubMed

  Regulation

  • expressed late during sporulation in the mother cell (SigK) PubMed

  Sigma factors

  • SigK: sigma factor, PubMed, in sigK regulon

Biological materials

Mutant

• BKE16740 (spoVFB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTTTCCTTTTAATGACGACA,  downstream forward: _UP4_TAAATTTACAGAAAATCTTT
• BKK16740 (spoVFB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTTTCCTTTTAATGACGACA,  downstream forward: _UP4_TAAATTTACAGAAAATCTTT

References

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