spoVFA

168

dipicolinate synthase (subunit A)

Locus: BSU_16730

Molecular weight: 31.80 kDa

Isoelectric point: 5.89

Protein length: 297 aa

Gene length: 894 bp

Function: dipicolic acid production

Product: dipicolinate synthase (subunit A)

Essential: no

E.C.: null

Synonyms: spoVFA, dpaA

Genomic Context

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List of homologs in different organisms, belongs to COG5842 (Galperin et al., 2021)

Gene

Coordinates: 1,744,367  1,745,260

The protein

Catalyzed reaction/ biological activity

• (S)-2,3-dihydrodipicolinate + NADP⁺ --> dipicolinate + H⁺ + NADPH (according to UniProt)

Structure

• 2RIR (PDB)
• Q04809 (AlphaFold)

Expression and Regulation

Operons

• Genes: spoVFA-spoVFB-asd-dapG-dapA

  Description: PubMed

  Regulation

  • expressed late during sporulation in the mother cell (SigK) PubMed

  Sigma factors

  • SigK: sigma factor, PubMed, in sigK regulon

Biological materials

Mutant

• BKE16730 (spoVFA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTCTAAACGTTCACCTTC,  downstream forward: _UP4_CAAGCTGAGGAGGGGAAATA
• BKK16730 (spoVFA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTCTAAACGTTCACCTTC,  downstream forward: _UP4_CAAGCTGAGGAGGGGAAATA

References

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