codV

168

site-specific integrase/recombinase, partitioning of the terminus region after replication

Locus: BSU_16140

Molecular weight: 35.19 kDa

Isoelectric point: 9.31

Protein length: 304 aa

Gene length: 915 bp

Function: chromosome partitioning

Product: site-specific integrase/recombinase

Essential: no

Synonyms: codV

Genomic Context

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List of homologs in different organisms, belongs to COG4974 (Galperin et al., 2021)

Gene

Coordinates: 1,687,187  1,688,101

The protein

Catalyzed reaction/ biological activity

• resolution of chromosome dimers by site specific recombination at the dif site (located close to the terminus region on the chromosome)

Protein family

• phage integrase family (according to UniProt)

Structure

• 1A0P (PDB) (from E. coli, 39% identity, complex with XerD) PubMed
• P39776 (AlphaFold)

Paralogous protein(s)

• XerD

Localization

• cytoplasm (according to Swiss-Prot)
• assembles on the chromosome at the dif site throughout the cell cycle PubMed

Expression and Regulation

Operons

• Genes: trmFO-codV-clpQ-clpY-codY

  Description: PubMed

  Regulation

  • repressed during growth in the presence of branched chain amino acids (CodY) PubMed

  Regulatory mechanism

  • CodY: repression, PubMed, in codY regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

  Additional information

  • the intracellular concentration of CodY is about 2.5 myM (according to PubMed)

Biological materials

Mutant

• 1A794 ( codV::kan), PubMed, available at BGSC
• BKE16140 (codV::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGCCTAATACCTCCCCAG,  downstream forward: _UP4_AAGAAAAATTAAAGGAGGCC
• BKK16140 (codV::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGCCTAATACCTCCCCAG,  downstream forward: _UP4_AAGAAAAATTAAAGGAGGCC

References

Reviews

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Original Publications

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