coaBC

168

coenzyme A biosynthesis bifunctional protein CoaBC; phosphopantothenoyl-cysteine synthetase/decarboxylase

Locus: BSU_15700

Molecular weight: 43.82 kDa

Isoelectric point: 6.01

Protein length: 406 aa

Gene length: 1221 bp

Function: biosynthesis of coenzyme A

Product: phosphopantothenoyl-cysteine synthetase/decarboxylase

Essential: yes

E.C.: 4.1.1.36

Synonyms: yloI

Genomic Context

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List of homologs in different organisms, belongs to COG0452 (Galperin et al., 2021)

Gene

Coordinates: 1,642,851  1,644,071

Phenotypes of a mutant

• essential PubMed, becomes dispensable when coenzyme A salvage pathway is active (e.g. in presence of panteth(e)ine) PubMed

The protein

Catalyzed reaction/ biological activity

• H⁺ + N-4-phosphopantothenoyl-cysteine --> CO₂ + 4'-phosphopantetheine PubMed
• (R)-4'-phosphopantothenate + CTP + L-cysteine --> CMP + diphosphate + H⁺ + N-4-phosphopantothenoyl-cysteine PubMed

Protein family

• N-terminal part: HFCD (homo-oligomeric flavin containing Cys decarboxylase) superfamily (single member, according to UniProt)
• C-terminal part: PPC synthetase family (single member, according to UniProt)

Structure

• 6TGV (PDB) (from Mycobacterium smegmatis, 38.8% identity) PubMed
• 5INT (PDB) (from Bacillus anthracis, 60% identity in 52% C-terminal fragment)
• O35033 (AlphaFold)

Expression and Regulation

Operons

• Genes: yloC-remA-gmk-yloH-coaBC-priA-defA-fmt-yloM-yloN

  Description

  • PubMed

Biological materials

Mutant

• GP4670 (trpC2 ΔcoaBC::spc), available in Jörg Stülke's lab PubMed
• GP4681 (trpC2 ΔcoaBC::cat), available in Jörg Stülke's lab
• MGNA-B372 (yloI::erm), available at the NBRP B. subtilis, Japan

References

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