cysP

168

sulfate transport in via proton symport

Locus: BSU_15580

Molecular weight: 36.68 kDa

Isoelectric point: 10.27

Protein length: 354 aa

Gene length: 1065 bp

Function: sulfate uptake

Product: sulfate transport in via proton symport

Essential: no

E.C.: null

Synonyms: cysP, ylnA

Genomic Context

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List of homologs in different organisms, belongs to COG0306 (Galperin et al., 2021)

Gene

Coordinates: 1,631,095  1,632,159

The protein

Protein family

• inorganic phosphate transporter (PiT) (TC 2.A.20) family (with Pit, according to UniProt)

Structure

• O34734 (AlphaFold)

Localization

• cell membrane

Expression and Regulation

Operons

• Genes: cysH-cysP-sat-cysC-ylnD-sirB-ylnF

  Description: PubMed

  Regulation

  • induced by methionine starvation (S-box) PubMed
  • the S-box RNA is degraded by RNase Y PubMed

  Regulatory mechanism

  • CymR: transcription repression, [pubmed|], in cymR regulon
  • S-box: RNA switch, , the S-box riboswitch binds S-adenosylmethionine resulting in termination PubMed, in S-box

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• GP4479 (trpC2 ΔcysP::neo), available in Jörg Stülke's lab
• MGNA-B365 (ylnA::erm), available at the NBRP B. subtilis, Japan
• BKE15580 (cysP::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GGCTAATTCCATTTTGCAGC,  downstream forward: _UP4_TGATCATTAATCTGAGTAAT
• BKK15580 (cysP::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GGCTAATTCCATTTTGCAGC,  downstream forward: _UP4_TGATCATTAATCTGAGTAAT

Labs working on this gene/protein

• [[Isabelle Martin-Verstraete]], Institute Pasteur, Paris, France

References

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