YlmE

• Description: enzyme involved in controlling the availability of coenzyme A
  
  

Gene name	ylmE
Synonyms
Essential	no
Product	unknown
Function	control of the CoA pool
Gene expression levels in SubtiExpress: ylmE
MW, pI	25 kDa, 5.546
Gene length, protein length	690 bp, 230 aa
Immediate neighbours	ylmD, sepF
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

poorly characterized/ putative enzymes

This gene is a member of the following regulons

Spo0A regulon

The gene

Basic information

• Locus tag: BSU15380

Database entries

• BsubCyc: BSU15380

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • while the protein was described as an amino acid racemase important for biofilm formation PubMed, no racemase activity was detected in in vitro assays with a functionally active protein PubMed

• Protein family: UPF0001 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:
  • pyridoxal phosphate PubMed

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU15380

• Structure: 1W8G (the structure of YggS from E. coli 33% identity, 61% similarity)

• UniProt: O31727

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: ylmD-ylmE-sepF-ylmG-ylmH PubMed

• Expression browser: ylmE PubMed

• Sigma factor:

• Regulation:
  • repressed under conditions that trigger sporulation (Spo0A) PubMed

• Regulatory mechanism:
  • Spo0A: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Tomokazu Ito, Jumpei Iimori, Sayuri Takayama, Akihito Moriyama, Ayako Yamauchi, Hisashi Hemmi, Tohru Yoshimura
Conserved pyridoxal protein that regulates Ile and Val metabolism.
J Bacteriol: 2013, 195(24);5439-49
[PubMed:24097949] [WorldCat.org] [DOI] (I p)

Leendert W Hamoen, Jean-Christophe Meile, Wouter de Jong, Philippe Noirot, Jeff Errington
SepF, a novel FtsZ-interacting protein required for a late step in cell division.
Mol Microbiol: 2006, 59(3);989-99
[PubMed:16420366] [WorldCat.org] [DOI] (P p)

Virginie Molle, Masaya Fujita, Shane T Jensen, Patrick Eichenberger, José E González-Pastor, Jun S Liu, Richard Losick
The Spo0A regulon of Bacillus subtilis.
Mol Microbiol: 2003, 50(5);1683-701
[PubMed:14651647] [WorldCat.org] [DOI] (P p)