FtsL

• Description: cell-division protein (septum formation), controls together with EzrA dynamics of the FtsZ ring
  
  

Gene name	ftsL
Synonyms	ylxB, yllD
Essential	yes PubMed
Product	cell-division protein
Function	septum formation
Gene expression levels in SubtiExpress: ftsL
Interactions involving this protein in SubtInteract: FtsL
MW, pI	12 kDa, 10.083
Gene length, protein length	351 bp, 117 aa
Immediate neighbours	mraW, pbpB
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell division, essential genes, membrane proteins

The gene

Basic information

• Locus tag: BSU15150

Phenotypes of a mutant

essential PubMed

Database entries

• BsubCyc: BSU15150

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: ftsL family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity: degraded by RasP, stabilized against RasP cleavage by DivIC PubMed

• Interactions:
  • RasP-FtsL, DivIC-FtsL PubMed
  • PbpB-DivIB-(FtsL-DivIC)2 PubMed, as part of the divisome
  • WalK-FtsL PubMed

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• BsubCyc: BSU15150

• Structure:

• UniProt: Q07867

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: mraZ-mraW-ftsL-pbpB PubMed

• Expression browser: ftsL PubMed

• Sigma factor:

• Regulation:
  • constitutively expressed PubMed

• Regulatory mechanism:

• Additional information: FtsL is degraded by RasP PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Original Publications

Erik Nico Trip, Jan-Willem Veening, Eric J Stewart, Jeff Errington, Dirk-Jan Scheffers
Balanced transcription of cell division genes in Bacillus subtilis as revealed by single cell analysis.
Environ Microbiol: 2013, 15(12);3196-209
[PubMed:23701187] [WorldCat.org] [DOI] (I p)

Tatsuya Fukushima, Isako Furihata, Robyn Emmins, Richard A Daniel, James A Hoch, Hendrik Szurmant
A role for the essential YycG sensor histidine kinase in sensing cell division.
Mol Microbiol: 2011, 79(2);503-22
[PubMed:21219466] [WorldCat.org] [DOI] (I p)

Susan L Rowland, Kimberly D Wadsworth, Scott A Robson, Carine Robichon, Jon Beckwith, Glenn F King
Evidence from artificial septal targeting and site-directed mutagenesis that residues in the extracytoplasmic β domain of DivIB mediate its interaction with the divisomal transpeptidase PBP 2B.
J Bacteriol: 2010, 192(23);6116-25
[PubMed:20870765] [WorldCat.org] [DOI] (I p)

Inga Wadenpohl, Marc Bramkamp
DivIC stabilizes FtsL against RasP cleavage.
J Bacteriol: 2010, 192(19);5260-3
[PubMed:20644139] [WorldCat.org] [DOI] (I p)

Pamela Gamba, Jan-Willem Veening, Nigel J Saunders, Leendert W Hamoen, Richard A Daniel
Two-step assembly dynamics of the Bacillus subtilis divisome.
J Bacteriol: 2009, 191(13);4186-94
[PubMed:19429628] [WorldCat.org] [DOI] (I p)

Marc Bramkamp, Louise Weston, Richard A Daniel, Jeff Errington
Regulated intramembrane proteolysis of FtsL protein and the control of cell division in Bacillus subtilis.
Mol Microbiol: 2006, 62(2);580-91
[PubMed:17020588] [WorldCat.org] [DOI] (P p)

Richard A Daniel, Marie-Françoise Noirot-Gros, Philippe Noirot, Jeff Errington
Multiple interactions between the transmembrane division proteins of Bacillus subtilis and the role of FtsL instability in divisome assembly.
J Bacteriol: 2006, 188(21);7396-404
[PubMed:16936019] [WorldCat.org] [DOI] (P p)

Yoshikazu Kawai, Naotake Ogasawara
Bacillus subtilis EzrA and FtsL synergistically regulate FtsZ ring dynamics during cell division.
Microbiology (Reading): 2006, 152(Pt 4);1129-1141
[PubMed:16549676] [WorldCat.org] [DOI] (P p)

Scott A Robson, Katharine A Michie, Joel P Mackay, Elizabeth Harry, Glenn F King
The Bacillus subtilis cell division proteins FtsL and DivIC are intrinsically unstable and do not interact with one another in the absence of other septasomal components.
Mol Microbiol: 2002, 44(3);663-74
[PubMed:11994149] [WorldCat.org] [DOI] (P p)

J Sievers, J Errington
Analysis of the essential cell division gene ftsL of Bacillus subtilis by mutagenesis and heterologous complementation.
J Bacteriol: 2000, 182(19);5572-9
[PubMed:10986263] [WorldCat.org] [DOI] (P p)

J Sievers, J Errington
The Bacillus subtilis cell division protein FtsL localizes to sites of septation and interacts with DivIC.
Mol Microbiol: 2000, 36(4);846-55
[PubMed:10844672] [WorldCat.org] [DOI] (P p)

R A Daniel, J Errington
Intrinsic instability of the essential cell division protein FtsL of Bacillus subtilis and a role for DivIB protein in FtsL turnover.
Mol Microbiol: 2000, 36(2);278-89
[PubMed:10792716] [WorldCat.org] [DOI] (P p)

R A Daniel, E J Harry, V L Katis, R G Wake, J Errington
Characterization of the essential cell division gene ftsL(yIID) of Bacillus subtilis and its role in the assembly of the division apparatus.
Mol Microbiol: 1998, 29(2);593-604
[PubMed:9720875] [WorldCat.org] [DOI] (P p)

R A Daniel, A M Williams, J Errington
A complex four-gene operon containing essential cell division gene pbpB in Bacillus subtilis.
J Bacteriol: 1996, 178(8);2343-50
[PubMed:8636036] [WorldCat.org] [DOI] (P p)