ahpT

168

thiol disulfide oxidoreductase, reduces AhpA

Locus: BSU_14230

Molecular weight: 17.66 kDa

Isoelectric point: 6.33

Protein length: 148 aa

Gene length: 447 bp

Function: protection of proteins against oxidative damage

Product: thiol disulfide oxidoreductase

Essential: no

Synonyms: ahpT, ykuV

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0526 (Galperin et al., 2021)

Gene

Coordinates: 1,492,875  1,493,321

The protein

Domains

• Thioredoxin domain (aa 2-145) (according to UniProt)

Structure

• 2B5X (PDB) (reduced form),  2B5Y (PDB) (oxidized form)
• O31699 (AlphaFold)

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: ahpA-ahpT

  Description: PubMed

  Regulatory mechanism

  • AbrB: repression, PubMed, in abrB regulon

Biological materials

Mutant

• GP1729 ahpAT::kan trpC2 available in Jörg Stülke's lab
• MGNA-B344 (ykuV::erm), available at the NBRP B. subtilis, Japan
• BKE14230 (ahpT::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTGCTTTTTCCCTCCTAT,  downstream forward: _UP4_TAGGTATCTGACTAAATAGT
• BKK14230 (ahpT::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTGCTTTTTCCCTCCTAT,  downstream forward: _UP4_TAGGTATCTGACTAAATAGT

References

Loading