stoA

168

thiol-disulfide oxidoreductase, with thioredoxin-like domain, required for the synthesis of the endospore peptidoglycan cortex

Locus: BSU_13840

Molecular weight: 18.66 kDa

Isoelectric point: 5.3

Protein length: 165 aa

Gene length: 498 bp

Function: spore cortex formation, maturation of SpoVD in the forespore outer membrane

Product: thiol-disulfide oxidoreductase

Essential: no

Synonyms: stoA, spoIVH, ykvV

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG1225 (Galperin et al., 2021)

Gene

Coordinates: 1,450,638  1,451,135

The protein

Catalyzed reaction/ biological activity

• reduction of intramolecular disulfide bonds in SpoVD in the forespore outer membrane PubMed

Protein family

• Thioredoxin family (according to UniProt)

Domains

• Thioredoxin domain (aa 27-165) (according to UniProt)

Structure

• 3ERW (PDB) PubMed
• O31687 (AlphaFold)

Paralogous protein(s)

• YneN, ResA

Localization

• forespore envelope PubMed

Expression and Regulation

Operons

• Genes: ykvU-stoA

  Description: PubMed

  Regulation

  • expressed during sporulation (SigE, SigG) PubMed

  Sigma factors

  • SigE: sigma factor, PubMed, in sigE regulon
  • SigG: sigma factor, PubMed, in sigG regulon

  Additional information

  • A ncRNA is predicted between 'stoA' and 'zosA' PubMed

• Genes: stoA

  Description: PubMed

  Regulation

  • expressed during sporulation (SigE, SigG) PubMed

  Additional information

  • A ncRNA is predicted between 'stoA' and 'zosA' PubMed

Biological materials

Mutant

• MGNA-B328 (ykvV::erm), available at the NBRP B. subtilis, Japan
• 1S130 ( stoA::cat), PubMed, available at BGSC
• 1S131 ( stoA::tet), PubMed, available at BGSC
• BKE13840 (stoA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGGAATCTTCCTTTCATG,  downstream forward: _UP4_TAGCTGAGAGCATAGACTCT
• BKK13840 (stoA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGGAATCTTCCTTTCATG,  downstream forward: _UP4_TAGCTGAGAGCATAGACTCT

Labs working on this gene/protein

• Lars Hederstedt, University of Lund, Sweden  Homepage

References

Reviews

Loading

Original Publications

Loading