ykoH

168

two-component sensor kinase

Locus: BSU_13260

Molecular weight: 50.49 kDa

Isoelectric point: 6.17

Protein length: 454 aa

Gene length: 1365 bp

Function: unknown

Product: two-component sensor kinase

Essential: no

Synonyms: ykoH

Genomic Context

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List of homologs in different organisms, belongs to COG0642 (Galperin et al., 2021)

Gene

Coordinates: 1,392,643  1,394,007

The protein

Catalyzed reaction/ biological activity

• autophosphorylation, phosphorylation of YkoG
• ATP + protein L-histidine --> ADP + protein N-phospho-L-histidine (according to UniProt)

Domains

• two transmembrane segments
• HAMP domain (aa 176-230) (according to UniProt)
• Histidine kinase domain (aa 238-450) (according to UniProt)

Structure

• 5C93 (PDB) (Lactobacillus plantarum WalK, corresponds to aa 230 ... 441, 32% identity) PubMed
• O34638 (AlphaFold)

Modification

• autophosphorylation on a His residue

Paralogous protein(s)

• KinE, CssS, YclK, YvrG

Localization

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• Genes: ykoG-ykoH-ykoI-ykoJ

  Description: PubMed

Biological materials

Mutant

• MGNA-B312 (ykoH::erm), available at the NBRP B. subtilis, Japan
• BKE13260 (ykoH::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_AATCTTGGTCTTCAGCTTCA,  downstream forward: _UP4_AGCGAACAGAATGGGGGAGG
• BKK13260 (ykoH::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_AATCTTGGTCTTCAGCTTCA,  downstream forward: _UP4_AGCGAACAGAATGGGGGAGG

References

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