ykkD

168

putative guanidine exporter

Locus: BSU_13100

Molecular weight: 11.19 kDa

Isoelectric point: 8.62

Protein length: 105 aa

Gene length: 318 bp

Function: export/ detoxification of guanidine

Product: subunit of guanidine exporter

Essential: no

Synonyms: ykkD

Genomic Context

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Categories containing this gene/protein

Cellular processes, Transporters, Transporters/ other, Other exporters

Groups of genes, Membrane proteins

List of homologs in different organisms, belongs to COG2076 (Galperin et al., 2021)

This gene is a member of the following regulons

E9866855F0156859C45E98F3B385FE1263BB7EBC regulon

Gene

Coordinates: 1,376,855  1,377,172

The protein

Protein family

paired small multidrug resistance  protein family (PSMR family) PubMed
drug/metabolite transporter (DMT) superfamily (according to UniProt)
Small multidrug resistance (SMR) (TC 2.A.7.1) family (according to UniProt)

Structure

3B5D (PDB) (from E. coli, 29% identity) PubMed
P49857 (AlphaFold)

Localization

cell membrane (according to UniProt)

Expression and Regulation

Operons

Genes: ykkC-ykkD

Description: PubMed

Regulation

induced in the presence of guanidine (ykkC riboswitch) PubMed

Regulatory mechanism

YkkC riboswitch: RNA switch, PubMed, in YkkC riboswitch regulon

Biological materials

Mutant

MGNA-A748 (ykkD::erm), available at the NBRP B. subtilis, Japan
BKE13100 (ykkD::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAATAAACTGATCCAGTGCA,  downstream forward: _UP4_TAAATTGATTTTTATCAAAT
BKK13100 (ykkD::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAATAAACTGATCCAGTGCA,  downstream forward: _UP4_TAAATTGATTTTTATCAAAT

References

Reviews

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Original Publications

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