ykfA

168

similar to LD-carboxypeptidase; may cleave C-terminal D-Ala from peptidoglycan tetrapeptides

Locus: BSU_12970

Molecular weight: 25.26 kDa

Isoelectric point: 5.41

Protein length: 319 aa

Gene length: 960 bp

Function: unknown

Product: unknown

Essential: no

Synonyms: ykfA

Genomic Context

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Categories containing this gene/protein

Cellular processes, Cell envelope and cell division, Cell wall degradation/ turnover, Utilization of cell wall components

List of homologs in different organisms, belongs to COG1619 (Galperin et al., 2021)

This gene is a member of the following regulons

CodY regulon

Gene

Coordinates: 1,365,888 → 1,366,847

The protein

Protein family

peptidase S66 family (with YocD, according to UniProt)

Structure

3SR3 (PDB) (MccF from B. anthracis, 30% identity) PubMed
5Z01 (PDB) (LdcA from E. coli, 30% identity) PubMed
O34851 (AlphaFold)

Expression and Regulation

Operons

Genes: dppA-dppB-dppC-dppD-dppE-ykfA-ykfB-ykfC-ykfD

Description: PubMed

Regulatory mechanism

CodY: repression, PubMed, in codY regulon

Biological materials

Mutant

MGNA-A740 (ykfA::erm), available at the NBRP B. subtilis, Japan
BKE12970 (ΔykfA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTGCTCTGCACCCTACG,  downstream forward: _UP4_GTTTCAGAAGGGGCGCTGAA
BKK12970 (ΔykfA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTGCTCTGCACCCTACG,  downstream forward: _UP4_GTTTCAGAAGGGGCGCTGAA

References

Reviews

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Original Publications

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