dppD

168

dipeptide ABC transporter (ATP-binding protein)

Locus: BSU_12950

Molecular weight: 36.53 kDa

Isoelectric point: 7.52

Protein length: 335 aa

Gene length: 1008 bp

Function: uptake of dipeptides

Product: dipeptide ABC transporter (ATP-binding protein)

Essential: no

Synonyms: dppD, dciAD

Genomic Context

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List of homologs in different organisms, belongs to COG0444 (Galperin et al., 2021)

Gene

Coordinates: 1,363,141  1,364,148

The protein

Protein family

• ABC transporter superfamily (according to UniProt)

Domains

• ABC transporter domain (aa 7-256) (according to UniProt)

Structure

• 4FWI (PDB) (from Caldanaerobacter subterraneus, 38% identity) PubMed
• P26905 (AlphaFold)

Paralogous protein(s)

• YkfD, OppD, AppD

Localization

• attached to the cell membrane (via DppB-DppC) PubMed

Expression and Regulation

Operons

• Genes: dppA-dppB-dppC-dppD-dppE

  Description: PubMed

  Regulation

  • repressed by glucose (2.9-fold) PubMed

  Regulatory mechanism

  • CodY: repression, PubMed, in codY regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

• Genes: dppA-dppB-dppC-dppD-dppE-ykfA-ykfB-ykfC-ykfD

  Description: PubMed

  Regulatory mechanism

  • CodY: repression, PubMed, in codY regulon

Additional information

• translation is likely to require Efp due to the presence of several consecutive proline residues PubMed

Biological materials

Mutant

• BKE12950 (dppD::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GACTGACAGAACTTTTTCCA,  downstream forward: _UP4_TCAGGAGATGCGAAGGATTG
• BKK12950 (dppD::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GACTGACAGAACTTTTTCCA,  downstream forward: _UP4_TCAGGAGATGCGAAGGATTG

References

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