pit

168

low-affinity inorganic phosphate transporter, proton symporter

Locus: BSU_12840

Molecular weight: 34.63 kDa

Isoelectric point: 10.45

Protein length: 333 aa

Gene length: 1002 bp

Function: phosphate uptake

Product: low-affinity inorganic phosphate transporter, proton symporter

Essential: no

Synonyms: pit, ykaB

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0306 (Galperin et al., 2021)

Gene

Coordinates: 1,349,468 → 1,350,469

The protein

Protein family

• inorganic phosphate transporter (PiT) (TC 2.A.20) family (with CysP, according to UniProt)

Structure

• O34436 (AlphaFold)

Localization

• membrane associated PubMed

Expression and Regulation

Operons

• Genes: ykaA-pit

  Description: PubMed

  Regulation

  • repressed under conditions that trigger sporulation (Spo0A) PubMed

  Regulatory mechanism

  • Spo0A: repression, PubMed, in spo0A regulon

Biological materials

Mutant

• MGNA-A735 (pit::erm), available at the NBRP B. subtilis, Japan
• BKE12840 (Δpit::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TAGAATATCCATTTTTACCC,  downstream forward: _UP4_TAATGTCACACCGCTTCTGT
• BKK12840 (Δpit::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TAGAATATCCATTTTTACCC,  downstream forward: _UP4_TAATGTCACACCGCTTCTGT

References

Reviews

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Original Publications

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