pit

168

low-affinity inorganic phosphate transporter, proton symporter

Locus: BSU_12840

Molecular weight: 34.63 kDa

Isoelectric point: 10.45

Protein length: 333 aa

Gene length: 1002 bp

Function: phosphate uptake

Product: low-affinity inorganic phosphate transporter, proton symporter

Essential: no

Synonyms: pit, ykaB

Genomic Context

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Categories containing this gene/protein

Cellular processes, Transporters, Transporters/ other, Uptake of other small ions

Metabolism, Additional metabolic pathways, Phosphate metabolism

Groups of genes, Membrane proteins

List of homologs in different organisms, belongs to COG0306 (Galperin et al., 2021)

This gene is a member of the following regulons

Spo0A regulon

Gene

Coordinates: 1,349,468 → 1,350,469

The protein

Protein family

inorganic phosphate transporter (PiT) (TC 2.A.20) family (with CysP, according to UniProt)

Structure

O34436 (AlphaFold)

Localization

membrane associated PubMed

Expression and Regulation

Operons

Genes: ykaA-pit

Description: PubMed

Regulation

repressed under conditions that trigger sporulation (Spo0A) PubMed

Regulatory mechanism

Spo0A: repression, PubMed, in spo0A regulon

Biological materials

Mutant

MGNA-A735 (pit::erm), available at the NBRP B. subtilis, Japan
BKE12840 (Δpit::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TAGAATATCCATTTTTACCC,  downstream forward: _UP4_TAATGTCACACCGCTTCTGT
BKK12840 (Δpit::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TAGAATATCCATTTTTACCC,  downstream forward: _UP4_TAATGTCACACCGCTTCTGT

References

Reviews

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Original Publications

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