xlyB

168

N-acetylmuramoyl-L-alanine amidase

Locus: BSU_12460

Molecular weight: 33.71 kDa

Isoelectric point: 9.8

Protein length: 317 aa

Gene length: 954 bp

Function: PBSX prophage-mediated lysis

Product: N-acetylmuramoyl-L-alanine amidase

Essential: no

E.C.: 3.5.1.28

Synonyms: xlyB, yjpB

Genomic Context

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Categories containing this gene/protein

Cellular processes, Cell envelope and cell division, Cell wall degradation/ turnover, Autolysis

Prophages and mobile genetic elements, Prophages, PBSX prophage

Prophages and mobile genetic elements, Prophages, Phage-related functions

Groups of genes, Secreted proteins

List of homologs in different organisms, belongs to COG5632 (Galperin et al., 2021)

Gene

Coordinates: 1,317,535  1,318,488

The protein

Catalyzed reaction/ biological activity

Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides (according to UniProt)

Protein family

N-acetylmuramoyl-L-alanine amidase 2 family (according to UniProt)

Domains

contains a N-acetylglucosamine-polymer-binding LysM domain PubMed
contains an amidase_2 domain (like BlyA, CwlA, CwlH, XlyB)
N-acetylmuramoyl-L-alanine amidase domain (aa 40-142) (according to UniProt)
LysM domain (aa 177-221) (according to UniProt)

Structure

2L47 (PDB) (from Bacillus phage Gamma, corresponds to aa 3 ... 166, 57% identity)
O34391 (AlphaFold)

Paralogous protein(s)

CwlA, CwlH, XlyA

Localization

secreted (according to UniProt)

Expression and Regulation

Operons

Genes: xlyB

Description: PubMed

Additional information

the mRNA is very stable (> 15 min) PubMed

Biological materials

Mutant

BKE12460 (xlyB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACCTATCATCTCTCCTTTT,  downstream forward: _UP4_TAAATGAAAAGACGGAAGCT
BKK12460 (xlyB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACCTATCATCTCTCCTTTT,  downstream forward: _UP4_TAAATGAAAAGACGGAAGCT

References

Reviews

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Original Publications

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