uxaB

168

tagaturonate reductase

Locus: BSU_12380

Molecular weight: 55.19 kDa

Isoelectric point: 5.06

Protein length: 480 aa

Gene length: 1443 bp

Function: hexuronate utilization

Product: tagaturonate reductase

Essential: no

E.C.: 1.1.1.58

Synonyms: uxaB, yjmI

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0246 (Galperin et al., 2021)

Gene

Coordinates: 1,309,880  1,311,322

The protein

Catalyzed reaction/ biological activity

• D-altronate + NAD⁺ --> keto-D-tagaturonate + NADH (according to UniProt)

Protein family

• mannitol dehydrogenase family (with MtlD, according to UniProt)

Structure

• 4IM7 (PDB) (YdfI from E. coli, aa 1 - 370, 26% identity)
• O34354 (AlphaFold)

Expression and Regulation

Operons

• Genes: yjmC-yjmD-uxuA-yjmF-exuT-exuR-uxaB-uxaA

  Description: PubMed

  Regulation

  • induced by galacturonate (ExuR) PubMed

  Regulatory mechanism

  • ExuR: repression, in the absence of inducer, in exuR regulon
  • CcpA: repression, PubMed PubMed, in ccpA regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon
  • SigE: sigma factor, PubMed, in sigE regulon

• Genes: uxaC-yjmB-yjmC-yjmD-uxuA-yjmF-exuT-exuR-uxaB-uxaA

  Description: PubMed

  Regulation

  • induced by galacturonate (ExuR) PubMed

  Regulatory mechanism

  • ExuR: repression, in the absence of inducer, in exuR regulon
  • CcpA: repression, PubMed PubMed, in ccpA regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• MGNA-A373 (yjmI::erm), available at the NBRP B. subtilis, Japan
• BKE12380 (uxaB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACCCTTATTGCCTCCCTCT,  downstream forward: _UP4_TGCATACAAGGGGGAGAGGT
• BKK12380 (uxaB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACCCTTATTGCCTCCCTCT,  downstream forward: _UP4_TGCATACAAGGGGGAGAGGT

References

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