exuT

168

galacturonate transport in via proton symport, glucuronate transport in via proton symport

locus: BSU_12360

Molecular weight: 45.15 kDa

pI: 9.61

Protein length: 422 aa

Gene length: 1269 bp

function: hexuronate uptake

product: hexuronate transporter

essential: no

synonyms: exuT, yjmG

List of homologs in different organisms, belongs to COG2271 (Galperin et al., 2021)

Gene

Coordinates: 1,307,446  1,308,714

The protein

Protein family

• [wiki|major facilitator superfamily] (according to UniProt)

Structure

• [PDB|6E9N] (from E. coli, 30% identity) [pubmed|31083648]
• [AF|O34456]

[wiki|Localization]

• cell membrane (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|3AEA5D769B89E676C916FD8934D762B4C9A02AA2|yjmC]-[gene|CC55984E31C5698668CCCD4AC6E2210B2B41ACAB|yjmD]-[gene|A0A6D36BEC397345A1D17E6A7C34E1F612FD46C7|uxuA]-[gene|B886E504A767343042DE9BF4E23E6798D124A2CF|yjmF]-[gene|6D08E897DD697523FD0720704A25AD4472075B6C|exuT]-[gene|69B143987DCF73B5D080E3FD87A6CA2940E07DA8|exuR]-[gene|A1C64447E0074B06C9210CC51680273FAAE703FA|uxaB]-[gene|ED29D2DE48AADEA09C90F08B6021555C357946B4|uxaA]

  description: [Pubmed|9882655]

  regulation

  • induced by galacturonate ([protein|search|ExuR]) [Pubmed|9882655]

  regulatory mechanism

  • [protein|69B143987DCF73B5D080E3FD87A6CA2940E07DA8|exuR]: repression, in the absence of inducer, in [regulon|protein:69B143987DCF73B5D080E3FD87A6CA2940E07DA8|exuR regulon]
  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]: repression, [pubmed|9882655] [pubmed|10666464], in [regulon|protein:E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA regulon]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|9882655], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
  • [protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]: sigma factor, [Pubmed|15699190,9882655], in [regulon|protein:3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE regulon]

• genes: [gene|5A0C29C7E218701727523885D01A48D02266B421|uxaC]-[gene|59CD3E4C24326542D9C85BB6FE64FBF49869EB55|yjmB]-[gene|3AEA5D769B89E676C916FD8934D762B4C9A02AA2|yjmC]-[gene|CC55984E31C5698668CCCD4AC6E2210B2B41ACAB|yjmD]-[gene|A0A6D36BEC397345A1D17E6A7C34E1F612FD46C7|uxuA]-[gene|B886E504A767343042DE9BF4E23E6798D124A2CF|yjmF]-[gene|6D08E897DD697523FD0720704A25AD4472075B6C|exuT]-[gene|69B143987DCF73B5D080E3FD87A6CA2940E07DA8|exuR]-[gene|A1C64447E0074B06C9210CC51680273FAAE703FA|uxaB]-[gene|ED29D2DE48AADEA09C90F08B6021555C357946B4|uxaA]

  description: [Pubmed|9882655]

  regulation

  • induced by galacturonate ([protein|search|ExuR]) [Pubmed|9882655]

  regulatory mechanism

  • [protein|69B143987DCF73B5D080E3FD87A6CA2940E07DA8|exuR]: repression, in the absence of inducer, in [regulon|protein:69B143987DCF73B5D080E3FD87A6CA2940E07DA8|exuR regulon]
  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]: repression, [pubmed|9882655] [pubmed|10666464], in [regulon|protein:E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA regulon]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|9882655], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]

Biological materials

Mutant

• MGNA-A371 (yjmG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/371 NBRP B. subtilis, Japan]
• BKE12360 ([gene|6D08E897DD697523FD0720704A25AD4472075B6C|exuT]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE12360 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCTCAACCTCACCCCTAT,  downstream forward: _UP4_TAATCTGGAAATATAGTGGG
• BKK12360 ([gene|6D08E897DD697523FD0720704A25AD4472075B6C|exuT]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK12360 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCTCAACCTCACCCCTAT,  downstream forward: _UP4_TAATCTGGAAATATAGTGGG

References

• 9579062,9882655,10666464,31083648