exuT

168

galacturonate transport in via proton symport, glucuronate transport in via proton symport

Locus: BSU_12360

Molecular weight: 45.15 kDa

Isoelectric point: 9.61

Protein length: 422 aa

Gene length: 1269 bp

Function: hexuronate uptake

Product: hexuronate transporter

Essential: no

Synonyms: exuT, yjmG

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG2271 (Galperin et al., 2021)

Gene

Coordinates: 1,307,446  1,308,714

The protein

Protein family

• major facilitator superfamily (according to UniProt)

Structure

• 6E9N (PDB) (from E. coli, 30% identity) PubMed
• O34456 (AlphaFold)

Localization

• cell membrane (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: yjmC-yjmD-uxuA-yjmF-exuT-exuR-uxaB-uxaA

  Description: PubMed

  Regulation

  • induced by galacturonate (ExuR) PubMed

  Regulatory mechanism

  • ExuR: repression, in the absence of inducer, in exuR regulon
  • CcpA: repression, PubMed PubMed, in ccpA regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon
  • SigE: sigma factor, PubMed, in sigE regulon

• Genes: uxaC-yjmB-yjmC-yjmD-uxuA-yjmF-exuT-exuR-uxaB-uxaA

  Description: PubMed

  Regulation

  • induced by galacturonate (ExuR) PubMed

  Regulatory mechanism

  • ExuR: repression, in the absence of inducer, in exuR regulon
  • CcpA: repression, PubMed PubMed, in ccpA regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• MGNA-A371 (yjmG::erm), available at the NBRP B. subtilis, Japan
• BKE12360 (exuT::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCTCAACCTCACCCCTAT,  downstream forward: _UP4_TAATCTGGAAATATAGTGGG
• BKK12360 (exuT::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCTCAACCTCACCCCTAT,  downstream forward: _UP4_TAATCTGGAAATATAGTGGG

References

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