forC1

168

formate:menadione oxidoreductase (with ForE1), catalytic subunit, general stress protein, important for survival of salt and ethanol stress

Locus: BSU_12160

Molecular weight: 109.58 kDa

Isoelectric point: 5.18

Protein length: 985 aa

Gene length: 2958 bp

Function: unknown

Product: formate:menadione oxidoreductase

Essential: no

Synonyms: yjgC

Genomic Context

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List of homologs in different organisms, belongs to COG3383 (Galperin et al., 2021)

Gene

Coordinates: 1,285,591 → 1,288,548

The protein

Protein family

• C-terminal part: prokaryotic molybdopterin-containing oxidoreductase family (according to UniProt)

Domains

• 2Fe-2S ferredoxin-type domain (aa 3-79) (according to UniProt)
• 4Fe-4S His(Cys)3-ligated-type domain (aa 79-119) (according to UniProt)
• 2 4Fe-4S ferredoxin-type domains (aa 139-170, aa 182-211) (according to UniProt)
• 4Fe-4S Mo/W bis-MGD-type domain (aa 258-314) (according to UniProt)

Cofactors

• 4 4Fe-4S cluster PubMed
• 1 2FE-2S cluster PubMed
• molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) PubMed
• menachinone-7 PubMed

Structure

• 6TG9 (PDB) (from Rhodobacter capsulatus, 35% identity) PubMed
• 2FUG (PDB) (from Thermus thermophilus, 23% identity) PubMed
• O34720 (AlphaFold)

Paralogous protein(s)

• ForC2

Expression and Regulation

Operons

• Genes: forC1-forE1

  Description: PubMed

  Regulation

  • induced by stress (SigB) PubMed

  Sigma factors

  • SigB: sigma factor, PubMed, in sigB regulon

Biological materials

Mutant

• MGNA-A354 (yjgC::erm), available at the NBRP B. subtilis, Japan
• BKE12160 (ΔforC1::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCTCCATTCCCCTTTCTT,  downstream forward: _UP4_GCTGTGAAAAGGGGGATGGG
• BKK12160 (ΔforC1::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCTCCATTCCCCTTTCTT,  downstream forward: _UP4_GCTGTGAAAAGGGGGATGGG

References

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